would I loose the unlock?

No. So, don't worry about it.

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But don't choose the Activation/Youtube patch, as this results in a "No Signal" message.

where IS that option? I can't seem to find it in QuickPwn RC3 for windows

Use the Winpwn GUI

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sorry, posted this in the wrong section.... so can winpwn quickpwn my iphone? like without overwriting the hole OS?

I have a factory unlocked iphone.

You can use both winpwn or quickpwn.

In winpwn unclick the activate option. Just let quickpwn run.

After both have run the phone connects to the itunes server and hey presto the phone is still unlocked.

If you winpwn with the activate option ticked you get no service as the other threads have stated.

Dont worry you will still be unlocked.